Pearson Edexcel International GCSE in Biology · 4BI1 · Free · no account needed

Enzymes and pH — exam questions

9 original Incandio exam-style questions on enzymes and pH, 19 marks, about 19 minutes of writing. Each one carries the command word, the tariff and the specification statement it assesses, exactly as a paper presents them. This is an original Incandio practice question, written to reflect the structure and demands of the qualification. It is not an official Pearson Edexcel past-paper question.

This is the practice set for Enzymes and pH — the lesson that teaches it. Read the lesson first if the idea is new; come here when you want to find out whether you can turn it into marks. Completely free, no account needed to read.

What these questions ask of you

7 command words run through this set, and on Pearson Edexcel International GCSE Biology each one means something precise. Answering the wrong command is the commonest way a candidate who understands the lesson still loses the mark.

Which (1 mark)
one option chosen from the set given
Name (1–2 marks)
the correct term, spelled recognisably
State (1 mark)
one precise fact, no justification
Explain (2–4 marks)
a causal chain — WHY it happens, each link a separate marking point Misread as: describing what happens without giving the reason it happens
Describe (2–4 marks)
developed, linked statements of WHAT happens — no reason required, but detail is Misread as: giving one bare statement where the tariff asked for a developed account
Suggest (1–3 marks)
a plausible answer applying taught biology to a situation not taught
Give (1–2 marks)
a short factual answer drawn from recall or from the data

Assessed on Paper 1 (4BI1/1B) and Paper 2 (4BI1/2B), against AO1, AO2, AO3.

Where these sit in the specification

Pearson Edexcel International GCSE Biology (4BI1) — statements 2.13 · 2.14. Incandio is aligned to this specification; it is not published by, endorsed by or affiliated with Pearson, and it reproduces none of Pearson's wording.

The 9 questions

Answer them on paper, then open the lesson's exam practice to have them marked. The mark scheme, the accepted and rejected answers and the annotated model answer are in Incandio and are not printed here — an answer beside its question is not practice.

  1. Figure 1 — the rate of reaction of enzyme X at different pH values. Plotted as a line; the graph is drawn in Incandio and the readings are below.
    pHRate of reaction / arbitrary units
    20
    32
    48
    520
    638
    746
    834
    912
    101
    Figure 2 — the rate of reaction of enzyme Y at different pH values. Plotted as a line; the graph is drawn in Incandio and the readings are below.
    pHRate of reaction / arbitrary units
    114
    241
    330
    412
    53
    60
    70

    (a)

    (1)Give

    Give the optimum pH of enzyme X, using Figure 1.

    Figure 1 — the rate of reaction of enzyme X at different pH values. Plotted as a line; the graph is drawn in Incandio and the readings are below.
    pHRate of reaction / arbitrary units
    20
    32
    48
    520
    638
    746
    834
    912
    101
    Figure 2 — the rate of reaction of enzyme Y at different pH values. Plotted as a line; the graph is drawn in Incandio and the readings are below.
    pHRate of reaction / arbitrary units
    114
    241
    330
    412
    53
    60
    70

    optimum pH of enzyme X = ............................

    Specification 2.13 · AO3 · about 1 min

    (b)

    (3)Explain

    Explain the shape of the curve for enzyme X between pH 7 and pH 10.

    Figure 1 — the rate of reaction of enzyme X at different pH values. Plotted as a line; the graph is drawn in Incandio and the readings are below.
    pHRate of reaction / arbitrary units
    20
    32
    48
    520
    638
    746
    834
    912
    101
    Figure 2 — the rate of reaction of enzyme Y at different pH values. Plotted as a line; the graph is drawn in Incandio and the readings are below.
    pHRate of reaction / arbitrary units
    114
    241
    330
    412
    53
    60
    70

    Specification 2.13 · AO3 · about 3 min

    (c)

    (2)Suggest

    Suggest where in the human digestive system enzyme Y is most likely to act, using Figure 2.

    Figure 1 — the rate of reaction of enzyme X at different pH values. Plotted as a line; the graph is drawn in Incandio and the readings are below.
    pHRate of reaction / arbitrary units
    20
    32
    48
    520
    638
    746
    834
    912
    101
    Figure 2 — the rate of reaction of enzyme Y at different pH values. Plotted as a line; the graph is drawn in Incandio and the readings are below.
    pHRate of reaction / arbitrary units
    114
    241
    330
    412
    53
    60
    70

    Specification 2.13 · AO2 · about 2 min

    (Total for Question 1 = 6 marks)

  2. (1)Which

    Which pH is the optimum for the protease released into the human stomach?

    pH
    ApH 2
    BpH 7
    CpH 9
    DpH 12

    Specification 2.13 · AO1 · about 1 min

    (Total for Question 2 = 1 mark)

  3. (1)Name

    Name the process by which an enzyme permanently loses its shape at an extreme pH.

    Specification 2.13 · AO1 · about 1 min

    (Total for Question 3 = 1 mark)

  4. (1)State

    State what is meant by the optimum pH of an enzyme.

    Specification 2.13 · AO1 · about 1 min

    (Total for Question 4 = 1 mark)

  5. (4)Explain

    Explain why an enzyme stops working when the pH is moved far below its optimum.

    Specification 2.13 · AO1 + AO2 · about 4 min

    (Total for Question 5 = 4 marks)

  6. (3)Describe

    Describe how a buffer solution would be used in an investigation into the effect of pH on an enzyme, and why it is needed.

    Specification 2.14 · AO3 · about 3 min

    (Total for Question 6 = 3 marks)

  7. (3)Suggest

    An enzyme extracted from bacteria living in an alkaline soda lake has an optimum of pH 10. Suggest why this enzyme is used in washing powders.

    Specification 2.13 · AO2 · about 3 min

    (Total for Question 7 = 3 marks)

Have them marked, then defend the answer

Practice tells you the mark; teaching it back tells you whether you understand it. On Incandio a topic counts as mastered only once you have taught it and defended it.

  1. Mark these questions — 9 questions, marked on Incandio against the authored scheme, with the mistake you actually made named.
  2. Teach it — explain enzymes and pH to Ember, an AI pupil who does not have it yet and asks the awkward question.
  3. Defend it — argue it against Søren Sørensen over three rounds, marked against descriptors you can read first.

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